Related Experiment Video
Updated: Aug 3, 2025
10:25
Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
5.0K
Alternative Splicing: From Abiotic Stress Tolerance to Evolutionary Genomics
Bei Gao1,2, Moxian Chen3, Melvin J Oliver4
1State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.
International Journal of Molecular Sciences
|April 13, 2023
Summary
Alternative splicing (AS) significantly increases the diversity of eukaryotic transcriptomes and proteomes. Understanding AS is crucial for deciphering gene expression complexity.
Area of Science:
- Molecular Biology
- Genetics
- Genomics
Background:
- Gene expression is regulated at multiple levels, including post-transcriptional modifications.
- Alternative splicing (AS) is a key mechanism generating transcriptomic and proteomic diversity in eukaryotes.
Discussion:
- AS events contribute significantly to the complexity of eukaryotic gene expression.
- The study of AS is essential for understanding cellular function and disease.
Key Insights:
- Post-transcriptional regulation, particularly AS, expands the coding potential of genomes.
- AS generates distinct mRNA isoforms from a single gene, leading to diverse protein functions.
Outlook:
- Further research into AS mechanisms can reveal novel therapeutic targets.
- Investigating AS patterns aids in understanding developmental processes and disease pathologies.
Related Concept Videos
02:18Alternative RNA Splicing
21.5K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.5K
01:32RNA Splicing
56.6K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.6K
02:32Exon Recombination
3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.6K
02:13Overview of Transposition and Recombination
15.9K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.9K
01:36Pre-mRNA Processing: RNA Splicing
5.3K
5.3K
01:59Plant Breeding and Biotechnology
19.5K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
19.5K