A PET Fluorescent Probe for Dynamic Pd²⁺ Tracking with Imaging Applications in the Nanofiber and Living Cells

Constructed on the moiety of a lactam screw ring, a near-infrared fluorescent probe RCya for Pd²⁺ was designed under the PET mechanism and synthesized by incorporating 2,4-dihydroxybenzaldehyde as the recognition group. Dynamic detection of aqueous Pd²⁺ by the probe RCya could be accomplished through ion competition, linear response, fluorescence-pH/time stabilities, and other optical tests. Moreover, the high selectivity, low cytotoxicity, cell permeability, and lysosome accumulation properties of RCya enabled the imaging applications on solid-state RCya-PAN composite nanofibers and in living cells. The recognition mechanism of probe RCya toward Pd²⁺ was further studied through simulation calculation and MS analysis.