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Compound 275# Induces Mitochondria-Mediated Apoptosis and Autophagy Initiation in Colorectal Cancer Cells through an

Dong-Lin Yang1,2, Yong Li1, Shui-Qing Ma1

  • 1College of Pharmacy, National & Local Joint Engineering Research Center of Targeted and Innovative Therapeutics, Chongqing Key Laboratory of Kinase Modulators as Innovative Medicine, Chongqing University of Arts and Sciences, Chongqing 402160, China.

Molecules (Basel, Switzerland)
|April 13, 2023
PubMed

Insights

A novel compound, N-(3-(5,7-dimethylbenzo [d]oxazol-2-yl)phenyl)-5-nitrofuran-2-carboxamide (compound 275#), effectively suppresses colorectal cancer (CRC) cell growth. It induces apoptosis and autophagy via reactive oxygen species (ROS) accumulation, showing therapeutic potential for CRC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Colorectal cancer (CRC) is a leading cause of cancer death, with a high rate of metastatic disease.
  • Effective therapeutic strategies for advanced CRC remain a critical unmet need.

Purpose of the Study:

  • To synthesize and evaluate a novel compound (compound 275#) for its antiproliferative activity against colorectal cancer cell lines.
  • To elucidate the molecular mechanisms underlying the anti-cancer effects of compound 275#.

Main Methods:

  • Synthesis of N-(3-(5,7-dimethylbenzo [d]oxazol-2-yl)phenyl)-5-nitrofuran-2-carboxamide (compound 275#).
  • Assessment of antiproliferative effects on CRC cell lines.
  • Analysis of apoptosis induction (caspase 3-mediated) and autophagy initiation.
  • Investigation of reactive oxygen species (ROS) levels and their role in the observed effects.

Main Results:

  • Compound 275# demonstrated significant antiproliferative capability against CRC cell lines.
  • Treatment with compound 275# induced caspase 3-mediated intrinsic apoptosis and autophagy.
  • The compound increased intracellular ROS accumulation, which mediated apoptosis and autophagy.

Conclusions:

  • Compound 275# exhibits potent anti-cancer properties against colorectal cancer.
  • ROS-mediated mitochondrial apoptosis and autophagy activation are key mechanisms of action for compound 275#.
  • Compound 275# represents a promising novel therapeutic candidate for CRC treatment.
Keywords:
CRCROSapoptosisautophagycompound 275#

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