Biogenic Synthesis, Characterization, and In Vitro Biological Evaluation of Silver Nanoparticles Using Cleome
Ayesha Ahmed Sumra1,2, Maryam Zain2, Tahira Saleem1
1Department of Chemistry, The Women University Multan, Multan 60000, Pakistan.
Plants (Basel, Switzerland)
|April 13, 2023
Summary
Green synthesized silver nanoparticles (Ag-NPs) from Cleome brachycarpa show potential as antidiabetic and antioxidant agents. These Ag-NPs also demonstrated anticancer activity against breast cancer cells, suggesting phytomedicine applications.
Area of Science:
- Nanotechnology
- Green Chemistry
- Pharmacology
Background:
- Silver nanoparticles (Ag-NPs) exhibit therapeutic potential.
- Plant-mediated synthesis offers a sustainable approach for Ag-NP production.
- Cleome brachycarpa extract can act as a reducing and stabilizing agent.
Purpose of the Study:
- To synthesize and characterize silver nanoparticles using Cleome brachycarpa aqueous extract.
- To evaluate the in vitro antioxidant, antidiabetic, and cytotoxic properties of the synthesized Ag-NPs.
- To explore the potential of these green synthesized Ag-NPs as phytomedicine.
Main Methods:
- Green synthesis of Ag-NPs using Cleome brachycarpa aqueous extract.
- Characterization via UV-Vis spectroscopy, AFM, FTIR, SEM, and EDX.
- In vitro assessment of antioxidant, antidiabetic, and anticancer (MCF-7 cell line) activities.
Main Results:
- Spherical Ag-NPs (20-80 nm) with even distribution were successfully synthesized.
- CB-AgNPs demonstrated significant antidiabetic potential at low concentrations.
- Antioxidant activity was observed across various concentrations, and anticancer activity against MCF-7 cells showed an IC50 of 18 μg/mL.
Conclusions:
- Green synthesized silver nanoparticles using Cleome brachycarpa are effective and eco-friendly.
- These CB-AgNPs show promise for managing diabetes and cancer therapeutics.
- Further investigation into their in vivo therapeutic attributes is warranted.
