Neutralization of alpha-bungarotoxin by monoclonal antibodies

We have produced monoclonal antibodies against alpha-bungarotoxin from Bungarus multicinctus, and controls were performed for their specificity and monoclonal character. The antibodies protected mice against the toxic effect of alpha-bungarotoxin for a few hours to six days, depending upon the chosen antibody (in comparison to a survival time of 79 min for the standard toxin dose). Antibodies can be grouped in several sets according to their duration of protection.