Polyurethane Application to Transform Screen-Printed Electrode for Rapid Identification of Histamine Isolated from...

Area of Science:

• Electrochemistry
• Materials Science
• Food Safety

Background:

• Histamine toxicity necessitates accurate determination methods.
• The FDA sets strict limits (50 mg·kg⁻¹) for histamine in food.
• Existing detection methods may lack simplicity or affordability for widespread use.

Purpose of the Study:

• To develop an innovative and reliable electrochemical sensor for histamine detection in fish.
• To create a molecularly imprinted polymer (MIP) based sensor on a screen-printed electrode (SPE).
• To validate the sensor's performance for accurate and precise histamine quantification.

Main Methods:

• Fabrication of a MIP-modified screen-printed electrode (MIP/SPE).
• Electrochemical characterization using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).
• Quantification of histamine using differential pulse voltammetry (DPV) across a linear range of 1-1000 nmol·L⁻¹.

Main Results:

• The MIP/SPE demonstrated a low detection limit of 1.765 nmol·L⁻¹ for histamine.
• The sensor exhibited a linear response within the tested concentration range.
• The developed electrochemical sensor provided accurate and precise histamine level determination in fish samples.

Conclusions:

• The MIP/SPE sensor offers a simple, inexpensive, and effective approach for histamine detection.
• This method is suitable for on-site analysis, enhancing food safety monitoring.
• The validated sensor meets the need for reliable histamine determination in fish products.