[Binding of noradrenaline with chromatin proteins from cell nuclei of the rat brain]

Peculiarities of binding of 1- and dl-norepinephrine preparations with chromatin proteins and incorporation of labelled amino acids into protein fractions of rat brain nuclei are studied in experiments in vivo and in vitro. It is established that the value of the label binding in the composition of norepinephrine with histones, nonhistone proteins and residual DNA-protein complex is directly proportional to the synthesis rate of the fractions under study. Single administration of ethanol increases the synthesis of nonhistone proteins and makes the level of norepinephrine binding with this fraction higher, Incorporation of amino acids and binding of norepinephrine with proteins of the residual DNA-protein complex in this case is, vice versa, inhibited. Ethanol administration does not change the synthesis of histones and binding of noradrenaline with it. An essential difference is observed in the intensity of 1- and dl-norepinephrine binding with protein fractions of brain nuclei. Interaction of d1-norepinephrine with karyoplasm protein is 7 times weaker than that of 1-isomer. The value of 1-norepinephrine binding with the residual DNA-protein complex in twice as high as d1-norepinephrine binding. Interaction of 1- and d1-norepinephrine preparations with histones and nonhistone protein differs insignificantly.