Related Experiment Video

Updated: Aug 2, 2025

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K

MicroRNA‑130a‑3p inhibition suppresses cervical cancer cell progression

Rhafaela Lima Causin1, André Van Helvoort Lengert1, Izabela Natalia Faria Gomes1

  • 1Molecular Oncology Research Center, Barretos Cancer Hospital, Teaching and Research Institute, São Paulo 14784‑400, Brazil.

Oncology Reports
|April 13, 2023
PubMed

Insights

MicroRNAs (miRNAs) drive cervical cancer growth. Inhibiting miR-130a-3p suppressed tumor cell proliferation, migration, and invasion, suggesting its potential as a biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are crucial in human tumor development, including cervical cancer.
  • The specific roles and mechanisms of miRNAs in cervical cancer progression are not fully understood.

Purpose of the Study:

  • To investigate the functional role of microRNA-130a-3p (miR-130a-3p) in cervical cancer.
  • To identify potential molecular targets of miR-130a-3p in cervical cancer cells.

Main Methods:

  • Cervical cancer cell lines (HeLa, SiHa, CaSki, C-4I, HCB-514) were used.
  • Cells were transfected with an inhibitor for miR-130a-3p (anti-miR-130a-3p).
  • Assays evaluated adhesion-independent cell proliferation, migration, and invasion.

Main Results:

  • miR-130a-3p was found to be overexpressed in multiple cervical cancer cell lines.
  • Inhibiting miR-130a-3p significantly decreased cervical cancer cell proliferation, migration, and invasion.
  • Delta-like Notch1 ligand (DLL1) was identified as a potential direct target of miR-130a-3p and was downregulated in cervical cancer tissues.

Conclusions:

  • miR-130a-3p promotes the proliferation, migration, and invasion of cervical cancer cells.
  • miR-130a-3p may serve as a valuable biomarker for assessing cervical cancer progression.
Keywords:
biomarkercervical cancermicroRNA‑130a‑3ptumor progression

More Related Videos

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.4K
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.4K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.2K