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Silencing circular RNA hsa_circABCC1 inhibits osteosarcoma progression through down-regulating HDAC4 via sponging

Kai Wang1, Na Wang2, Jingsheng Liu1

  • 1Department of Orthopedics, Second Hospital of Lanzhou University, Lanzhou, China.

Environmental Toxicology
|April 13, 2023
PubMed
Abstract

Insights

Circular RNA ABCC1 (circABCC1) promotes osteosarcoma (OS) progression by increasing HDAC4 expression via sponging miR-591. Silencing circABCC1 inhibits OS cell viability, proliferation, migration, and invasion while promoting apoptosis and autophagy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are crucial regulators in cancer development.
  • The specific role of circRNA ABCC1 (circABCC1) in osteosarcoma (OS) remains largely uncharacterized.
  • Understanding circABCC1's function is vital for developing novel OS therapeutic strategies.

Purpose of the Study:

  • To elucidate the role of circABCC1 in the progression of osteosarcoma.
  • To investigate the underlying molecular mechanisms of circABCC1 in OS.
  • To explore the potential of targeting circABCC1 for OS treatment.

Main Methods:

  • Quantitative real-time PCR and Western blot were used to measure circABCC1, miR-591, and HDAC4 expression in OS tissues and cells.
  • In vitro assays (CCK-8, EdU, FCM, Transwell) assessed cell viability, proliferation, apoptosis, migration, and invasion.
  • Dual luciferase reporter assays confirmed interactions between circABCC1/miR-591 and miR-591/HDAC4.
  • A tumor xenograft model evaluated the in vivo oncogenic role of circABCC1.

Main Results:

  • CircABCC1 expression was significantly upregulated in OS tissues and cells.
  • Silencing circABCC1 inhibited OS cell proliferation, viability, migration, and invasion, while promoting apoptosis and autophagy.
  • CircABCC1 acted as a sponge for miR-591, and miR-591 targeted HDAC4, forming the circABCC1/miR-591/HDAC4 axis.
  • Knockdown of circABCC1 suppressed tumor growth in vivo.

Conclusions:

  • CircABCC1 promotes osteosarcoma progression.
  • The circABCC1/miR-591/HDAC4 axis plays a critical role in OS development.
  • Silencing circABCC1 inhibits OS progression by downregulating HDAC4 expression via sponging miR-591.
Keywords:
HDAC4OScircABCC1miR-591

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