Function of environment-derived male perfumes in orchid bees

Perfume making in male orchid bees is a unique behavior that has given rise to an entire pollination syndrome in the neotropics.^1,2 Male orchid bees concoct and store species-specific perfume mixtures in specialized hind-leg pockets³ using volatiles acquired from multiple environmental sources, including orchid flowers.^4,5 However, the function and the ultimate causes of this behavior have remained elusive.^2,6 Although previous observations suggested that male perfumes serve as chemical signals, the attractiveness for females has not be shown.^7,8 Here, we demonstrate that the possession of perfume increases male mating success and paternity in Euglossa dilemma, a species of orchid bees recently naturalized in Florida. We supplemented males reared from trap-nests with perfume loads harvested from wild conspecifics. In dual-choice experiments, males supplemented with perfumes mated with more females, and sired more offspring, than untreated, equal-aged, control males. Although perfume supplementation had little effect on the intensity of male courtship display, it changed the dynamics of male-male interactions. Our results demonstrate that male-acquired perfumes are sexual signals that stimulate females for mating and suggest that sexual selection is key in shaping the evolution of perfume communication in orchid bees.