[Determination of free amino acids in body tissues]

The authors describe methods for isolation and assay of free amino acids in body tissues employed today in clinical and experimental studies. Give an account of the main factors affecting the data obtained. Compare the efficacy of different methods for quantitative determination of free amino acids in tissues. Analyse difficulties arising in the option of the deproteinizing agent while preparing tissue samples to be tested. Based on the reported data and their own experience the authors suggest efficacious and less time-consuming research methods for assay of free amino acids in blood plasma, red blood cells, and tissue homogenates.