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Apoptosis in physiological root resorption of primary teeth.
Y Turan1, N Akal2, B Yildirim3
1Department of Pediatric Dentistry, Faculty of Dentistry, University of Baskent, Ankara, Turkey.
Nigerian Journal of Clinical Practice
|April 14, 2023
Summary
Apoptosis, or programmed cell death, was investigated in deciduous teeth undergoing physiological root resorption. The study found no significant difference in apoptosis levels, suggesting it may not directly regulate pulp cell populations during this process.
Area of Science:
- Dentistry
- Cell Biology
- Histology
Background:
- Physiological root resorption of deciduous teeth involves collagen destruction and potentially apoptotic cell death.
- The precise mechanisms and sequence of events in pulp tissue cell death during physiological resorption remain unclear.
Purpose of the Study:
- To evaluate apoptotic cell death in deciduous teeth pulp during various stages of physiological root resorption.
- To investigate the role of apoptosis in pulp tissue elimination during deciduous tooth root resorption.
Main Methods:
- Extraction of healthy deciduous teeth with early and advanced root resorption (n=34) and healthy permanent teeth (n=12).
- Detection of apoptotic cells using the terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) method and transmission electron microscopy (TEM).
- Determination of apoptotic index (AI) by calculating the ratio of Apoptag-positive fibroblasts to total cells.
Main Results:
- No statistically significant differences in AI values were observed across different study groups (p>0.05).
- Apoptosis was identified in vascular endothelial cells, mononuclear inflammatory cells, and odontoblasts within the connective pulp tissue.
- TEM revealed pulp cells in various stages of apoptosis.
Conclusions:
- The similar AI values suggest apoptosis may regulate pulp cell populations independently of the physiological root resorption process in deciduous teeth.
- Apoptosis does not appear to be a primary driver for pulp tissue elimination during physiological root resorption.
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