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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Aug 2, 2025

A Sensitive Method to Quantify Senescent Cancer Cells

A Sensitive Method to Quantify Senescent Cancer Cells

Published on: August 2, 2013

Flow Cytometry-based Method for Efficient Sorting of Senescent Cells.

Erwan Goy¹, Nathalie Martin¹, Claire Drullion¹

¹Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, UMR9020-U1277 - CANTHER - Cancer Heterogeneity, Plasticity and Resistance to Therapies, F-59000 Lille, France.

|April 14, 2023

Summary

This summary is machine-generated.

This study introduces a new flow cytometry method to sort senescent cells based on three markers: SA-β-Gal activity, cell size, and granularity. This technique effectively separates senescent cells from proliferating ones, enabling detailed analysis of cellular senescence.

Keywords:

C12FDG FSC Flow cytometry Nozzle SA-β-Gal SSC Senescence Sorting

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Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

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Last Updated: Aug 2, 2025

A Sensitive Method to Quantify Senescent Cancer Cells

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Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

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Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells

Published on: July 12, 2022

Area of Science:

Cell Biology
Aging Research
Biotechnology

Background:

Cellular senescence is a stress-induced cell state linked to aging and disease.
Senescent cells accumulate with age and contribute to various pathologies.
Heterogeneity of senescent cell populations complicates research into senescence pathways.

Purpose of the Study:

To develop a flow cytometry strategy for isolating senescent cells.
To enable sorting of senescent cells based on multiple canonical markers.
To facilitate the study of senescence induction, maintenance, and escape mechanisms.

Main Methods:

Utilized flow cytometry with a novel strategy for senescent cell sorting.
Employed three canonical senescence markers: SA-β-Gal activity (C12FDG), cell size (FSC), and granularity (SSC).
Adapted sorting thresholds for independent marker detection and applied to human fibroblasts.

Main Results:

Developed an efficient protocol for sorting senescent cells from proliferating cells.
Demonstrated the need for large nozzle diameters (≥200 µm) for sorting large senescent cells.
Confirmed sorting efficiency, cell viability, and senescent phenotype of sorted cells using additional markers (p21, 53BP1 foci).

Conclusions:

The developed protocol enables the isolation of senescent cells with high purity.
Allows for the simultaneous sorting of senescent cell subpopulations with varying marker expression levels.
Provides a valuable tool for advancing research on cellular senescence and age-related diseases.