Antitumor Effects of Hydromorphone on Human Gastric Cancer Cells in vitro
Beiwei Liang1, Chunlai Li1, Zhan Zhou1
1Department of Anesthesiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, People's Republic of China.
Introduction:
Experimental data indicate that morphine and fentanyl may have antitumor effects in gastric cancer cells (GC). Hydromorphone, as an analgesic, is used against refractory cancer pain in recent years. However, the data on hydromorphone influencing the biological characteristics of human gastric cancer cells are lacking. The aim of this study was to investigate how hydromorphone affected the growth of human gastric cancer in vitro.
Material And Methods:
Human GC cell lines (HGC-27, MGC-803, AGS and SGC-7901) and human gastric epithelial cells GSE-1 were exposed to various concentrations of hydromorphone (0-800μM). The cell viability, invasion and migration abilities were measured using cell counting kit-8, Transwell and wound healing assays. Apoptosis and cell cycle were evaluated by flow cytometry.
Results:
Hydromorphone was toxic in GSE-1 cells at the concentration 800μM. It showed enhanced antitumor effects at a longer incubation time and higher concentrations in HGC-27, MGC-803, AGS and SGC-7901 cells. Hydromorphone inhibited the progression of MGC- 803 cells by cell cycle arrest and apoptosis induction.
Conclusion:
Hydromorphone suppresses the proliferation of human GC cells in a dose- and time-dependent manner. That may provide a theoretical basis for the clinical application of hydromorphone in the safe and effective treatment of GC.
Insights
Hydromorphone demonstrated antitumor effects against human gastric cancer (GC) cells in vitro. This study suggests hydromorphone may be a potential therapeutic agent for gastric cancer treatment.
Area of Science:
- Oncology
- Pharmacology
Background:
- Gastric cancer (GC) is a significant global health concern.
- Opioids like morphine and fentanyl show potential antitumor effects in GC.
- Data on hydromorphone's impact on human GC cells are limited.
Purpose of the Study:
- To investigate the effects of hydromorphone on the biological characteristics of human gastric cancer cells in vitro.
- To determine if hydromorphone influences the growth, viability, invasion, migration, apoptosis, and cell cycle of GC cells.
Main Methods:
- Human GC cell lines (HGC-27, MGC-803, AGS, SGC-7901) and normal gastric epithelial cells (GSE-1) were treated with varying hydromorphone concentrations (0-800μM).
- Cell viability, invasion, and migration were assessed using CCK-8, Transwell, and wound healing assays.
- Apoptosis and cell cycle progression were analyzed via flow cytometry.
Main Results:
- Hydromorphone exhibited toxicity in normal GSE-1 cells at 800μM.
- In GC cell lines, hydromorphone demonstrated enhanced antitumor effects with increased concentration and incubation time.
- Hydromorphone induced cell cycle arrest and apoptosis in MGC-803 cells, inhibiting their progression.
Conclusions:
- Hydromorphone suppresses human GC cell proliferation in a dose- and time-dependent manner.
- These findings provide a theoretical basis for the potential clinical application of hydromorphone in treating gastric cancer.
- Further research may explore hydromorphone's role in safe and effective GC therapy.
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