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Updated: Aug 2, 2025

Development of Compendium for Esophageal Squamous Cell Carcinoma
Published on: April 12, 2024
USP9X expression is functionally related to laryngeal cancer
Yu-Feng Wan1, Chen-Yu Zhang2, Xiao-Wen Cheng3,4,5
1Department of Otolaryngology-Head Neck Surgery, The Affiliated Chaohu Hospital of Anhui Medical University, Hefei, Anhui, 238001, P.R. China.
Ubiquitin-specific protease 9X (USP9X) is upregulated in laryngeal cancer, promoting tumor progression and metastasis. Inhibiting USP9X may offer a new therapeutic strategy for laryngeal cancer treatment.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- The role of USP9X in laryngeal cancer development is largely unknown.
- USP9X is implicated in various cancers, suggesting a potential role in laryngeal carcinogenesis.
Purpose of the Study:
- To investigate the expression and function of USP9X in laryngeal cancer.
- To determine if USP9X is a potential therapeutic target for laryngeal cancer.
Main Methods:
- Quantitative analysis of USP9X expression in laryngeal cancer tissues.
- Cell proliferation, migration, and invasion assays using USP9X knockdown in FaDu cells.
- Apoptosis assays and Western blot analysis to assess protein expression changes.
Main Results:
- USP9X expression is significantly upregulated in laryngeal cancer tissues.
- USP9X levels correlate with poor differentiation and lymphatic metastasis.
- USP9X knockdown inhibits proliferation, migration, and invasion while inducing apoptosis in FaDu cells.
- USP9X inhibition downregulates Mcl-1 and upregulates Bax protein expression.
Conclusions:
- USP9X plays a critical role in promoting laryngeal cancer occurrence and progression.
- USP9X is a potential biomarker for laryngeal cancer and a promising therapeutic target.
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