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RENEB Inter-Laboratory Comparison 2021: The FISH-Based Translocation Assay.

J-F Barquinero1, Y Abe2, N Aneva3

  • 1Universitat Autònoma de Barcelona (UAB), Barcelona, Spain.

Radiation Research
|April 14, 2023
PubMed
Summary
This summary is machine-generated.

This study assessed the accuracy of fluorescence in situ hybridization (FISH) for radiation dose assessment in an inter-laboratory comparison. While FISH effectively distinguished irradiated from non-irradiated samples, a tendency to overestimate radiation doses was observed.

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Area of Science:

  • Radiation biology
  • Cytogenetics
  • Biophysical dosimetry

Background:

  • Translocation analysis using fluorescence in situ hybridization (FISH) is crucial for assessing past ionizing radiation exposure.
  • Inter-laboratory comparisons for FISH-based dose assessment are less common than for other methods like dicentric analysis.
  • The European Network of Biological and Physical retrospective Dosimetry (RENEB) 2021 inter-laboratory comparison provided an opportunity to evaluate FISH performance.

Purpose of the Study:

  • To conduct an inter-laboratory comparison using the FISH technique for radiation dose assessment.
  • To gain experience with FISH-based dose assessment in a simulated emergency scenario.
  • To evaluate the consistency and accuracy of FISH translocation analysis across multiple laboratories.

Main Methods:

  • Peripheral blood samples from a healthy volunteer were irradiated with known doses (0, 1.2, 3.5 Gy) and sent to seven participating laboratories.
  • Laboratories applied their standard FISH techniques for translocation analysis.
  • Results were reported based on triage analysis (fewer cells) and full analysis (500 cells), with dose estimations provided.

Main Results:

  • Most laboratories successfully discriminated between unexposed and irradiated samples.
  • Triage analysis showed heterogeneity in cell counts, while full analysis was more homogeneous.
  • Excluding outlier results, mean dose estimations showed deviations from delivered doses, with a general tendency to overestimate.

Conclusions:

  • FISH is a viable method for discriminating irradiated from non-irradiated samples in retrospective dosimetry.
  • Harmonization efforts are needed to improve the accuracy and consistency of FISH-based dose assessment.
  • Further inter-laboratory exercises are recommended to address the observed overestimation of radiation doses.