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Published on: June 23, 2015
Urinary MicroRNAs, Possible Biomarkers for Early Detection of Patients with Autosomal Dominant Polycystic Kidney
Yaghoob Ghasemi, Alireza Mardomi, Effat Alizadeh
1Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. drtayebikh@yahoo.com.
Insights
Urinary microRNAs (miRs) show promise for early autosomal dominant polycystic kidney disease (ADPKD) diagnosis. This study found specific miR expression differences in asymptomatic ADPKD patients, suggesting their potential as biomarkers.
Area of Science:
- Nephrology
- Molecular Biology
- Biomarker Discovery
Background:
- Autosomal dominant polycystic kidney disease (ADPKD) is a common genetic kidney disorder characterized by renal epithelial cell overgrowth and cyst formation.
- Early diagnosis of ADPKD is crucial for disease management and preventing severe renal complications.
- MicroRNAs (miRs) are emerging as potential biomarkers due to their role in gene regulation and stability in biological fluids.
Purpose of the Study:
- To investigate the potential of urinary microRNAs (miRs) for the early diagnosis of autosomal dominant polycystic kidney disease (ADPKD) in asymptomatic individuals.
- To identify specific urinary miR expression profiles associated with early-stage ADPKD.
Main Methods:
- Quantitative real-time PCR (qRT-PCR) was used to analyze the expression of miR-17, miR-21, miR-143, and miR-223 in urine samples.
- Urine samples were collected from 20 asymptomatic ADPKD patients and 20 healthy controls.
- Serum kidney function markers were also assessed.
Main Results:
- Patients with ADPKD exhibited significantly higher urinary levels of miR-17, miR-21, and miR-143 compared to healthy controls.
- A lower level of miR-223 was observed in the urine of ADPKD patients.
- Differential expression patterns of these miRs were confirmed in ADPKD cases.
Conclusions:
- The study identified distinct urinary microRNA (miR) expression profiles in patients with autosomal dominant polycystic kidney disease (ADPKD).
- Urinary miRs may serve as valuable, non-invasive biomarkers for the early detection of ADPKD in asymptomatic individuals.
- Further research is warranted to validate these findings and establish clinical utility.
Introduction:
Autosomal dominant polycystic kidney disease (ADPKD) is a prevalent renal disorder that causes abnormal growth of renal epithelial cells. The excessive expansion of renal epithelial cells can lead to cyst formation that is associated with serious renal complications. The early diagnosis of ADPKD makes the control of the disease somehow attainable. Regarding the diagnostic potential of microRNAs (miRs) as robust clinical biomarkers, the present study aimed to examine the potential of urinary miRs in early diagnosis of ADPKD in asymptomatic patients.
Methods:
Urine samples were obtained from 20 asymptomatic ADPKD patients and 20 healthy control individuals and the miR content of the samples was extracted and converted to cDNA for the qRT-PCR experiment. The relative expressions of miR-17, miR-21, miR-143, and miR-223 were evaluated in ADPKD cases and healthy individuals. Serum levels of kidney function markers were also evaluated in the study participants.
Results:
The urine samples of patients with ADPKD demonstrated higher levels of miR-17, miR-21, and miR-143 along with a lower miR-223 level compared to the healthy control group.
Conclusion:
This study revealed the differential expression of the studied miRs in ADPKD patients. Detection of miRs in urinary samples might provide a useful platform for early diagnosis of ADPKD in asymptomatic patients. DOI: 10.52547/ijkd.7281.
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