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RefFinder: a web-based tool for comprehensively analyzing and identifying reference genes.

Fuliang Xie1, Jianyu Wang2, Baohong Zhang3

  • 1Department of Biology, East Carolina University, Greenville, NC, 27858, USA.

Functional & Integrative Genomics
|April 15, 2023
PubMed
Summary
This summary is machine-generated.

Selecting stable reference genes is crucial for accurate gene expression analysis. RefFinder is a web-based tool that integrates four algorithms to evaluate and rank candidate reference gene stability, ensuring reliable experimental results.

Keywords:
Gene expressionReference geneWeb-based toolqRT-PCR

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Area of Science:

  • Molecular Biology
  • Bioinformatics

Background:

  • Accurate gene expression analysis relies on stable reference genes.
  • No single reference gene is universally consistent across all conditions.
  • Identifying suitable reference genes is essential before expression studies.

Purpose of the Study:

  • To develop a comprehensive tool for evaluating reference gene stability.
  • To integrate multiple computational algorithms for robust ranking.
  • To provide a web-based platform for selecting reliable reference genes.

Main Methods:

  • Integration of geNorm, NormFinder, BestKeeper, and comparative ΔCt methods.
  • Development of a web-based tool (RefFinder) for comparative analysis.
  • Calculation of geometric mean of weights for final stability rankings.

Main Results:

  • RefFinder provides a unified platform for evaluating reference gene stability.
  • The tool allows selection of individual or combined algorithms for ranking.
  • It has been widely adopted and validated globally.

Conclusions:

  • RefFinder enhances the reliability of reference gene selection in expression studies.
  • The integrated approach offers a more robust evaluation than single methods.
  • This tool is valuable for researchers in molecular biology and related fields.