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Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

Constructing high-density tissue microarrays with a novel method and a self-made tissue-arraying instrument.

Ping Qin¹, Liu Li², Li Zhao³

¹Department of Pathology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510150, PR China; Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, PR China.

Pathology, Research and Practice

|April 15, 2023

Summary

This summary is machine-generated.

Researchers developed a novel method for constructing high-density tissue microarrays (TMAs) without spacing between tissue cores. This cost-effective technique enables simultaneous molecular marker analysis in numerous specimens, making TMA technology more accessible for all laboratories.

Keywords:

High-density Immunohistochemistry Novel method Tissue microarray

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Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas

Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas

Published on: May 31, 2012

Area of Science:

Biotechnology
Histology
Molecular Pathology

Background:

Tissue microarrays (TMAs) enable simultaneous molecular analysis of numerous tissue specimens under identical conditions.
Existing TMA construction methods often involve reducing tissue core diameter or spacing, with zero spacing being difficult to achieve without expensive equipment.
High-density TMAs are desirable for increased throughput in molecular pathology studies.

Purpose of the Study:

To introduce a novel, cost-effective method for constructing high-density tissue microarrays (TMAs) without inter-core spacing.
To present a method for creating a self-made, inexpensive tissue-arraying instrument for TMA construction.
To demonstrate the feasibility of the developed method for routine laboratory use.

Main Methods:

A novel technique was developed to construct tissue microarrays (TMAs) with adjacent tissue cores, eliminating spacing.
A simple, low-cost tissue-arraying instrument was fabricated using readily available materials.
The constructed TMA, containing 126 cores (2 mm diameter), was sectioned and stained using Hematoxylin and Eosin (H&E) and immunohistochemistry.

Main Results:

A high-density TMA with 126 tissue cores (2 mm diameter) was successfully constructed without any spacing between cores.
Hematoxylin and Eosin (H&E) staining and immunohistochemical staining on TMA sections showed no tissue spot loss.
The self-made tissue-arraying instrument proved effective and easy to operate.

Conclusions:

The novel method allows for the construction of high-density TMAs without spacing, enhancing efficiency in molecular analyses.
The inexpensive, self-made tissue-arraying instrument makes advanced TMA construction accessible to all laboratories.
This technique facilitates high-throughput molecular marker examination across numerous specimens, supporting broader research applications.