Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR Spectrometers01:25

IR Spectrometers

1.2K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
1.2K
IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

985
IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...
985
Infrared (IR) Spectroscopy: Overview01:09

Infrared (IR) Spectroscopy: Overview

2.0K
When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...
2.0K
IR Spectrum01:19

IR Spectrum

1.1K
When infrared (IR) radiation passes through a molecule, the bonds stretch or bend by absorbing the radiation. This absorption creates the molecule's absorption spectrum, which is the plot of its percentage transmittance versus wavenumber.
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0%...
1.1K
Methods of Classification and Identification01:28

Methods of Classification and Identification

63
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
63
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.0K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Occurrence and Antimicrobial Resistance of <i>Acinetobacter</i> spp. in Processing Environments of Slaughterhouses and Meat Processing Facilities.

Foods (Basel, Switzerland)·2026
Same author

Characterization of microbial diversity, chemical hazards and antimicrobial resistant bacteria in wash water from a fresh-cut vegetable processing plant.

International journal of food microbiology·2026
Same author

Microbiological safety of ungulates meat intended to be frozen and defrosting of frozen ungulates meat.

EFSA journal. European Food Safety Authority·2026
Same author

Update of the list of QPS-recommended biological agents intentionally added to food or feeds as notified to EFSA.

EFSA journal. European Food Safety Authority·2026
Same author

Update of the list of qualified presumption of safety (QPS) recommended microbiological agents intentionally added to food or feed as notified to EFSA 23: Suitability of taxonomic units notified to EFSA until September 2025.

EFSA journal. European Food Safety Authority·2026
Same author

Microbiome and resistome successions in pig carcasses and fresh pork meat throughout slaughtering, processing and shelf-life.

Microbiome·2026

Related Experiment Video

Updated: Aug 2, 2025

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

4.0K

Protocol for bacterial typing using Fourier transform infrared spectroscopy.

Huayan Yang1, Haimei Shi1, Bin Feng1

  • 1Department of Intensive Care Unit, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang 315211, China; Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China.

STAR Protocols
|April 16, 2023
PubMed
Summary
This summary is machine-generated.

Fourier transform infrared (FT-IR) spectroscopy provides specific bacterial identification. This study presents a unified protocol to improve FT-IR accuracy for bacterial typing, addressing sample preparation and data analysis challenges.

Keywords:
BioinformaticsBiophysicsCell BiologyCell cultureHealth SciencesMicrobiology

More Related Videos

Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments
04:07

Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments

Published on: February 23, 2024

1.4K
High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

33.3K

Related Experiment Videos

Last Updated: Aug 2, 2025

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry
08:51

Atomic Force Microscopy Combined with Infrared Spectroscopy as a Tool to Probe Single Bacterium Chemistry

Published on: September 15, 2020

4.0K
Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments
04:07

Author Spotlight: Integrated OPTIR-FISH for Single-Cell Metabolic and Identity Analysis in Complex Environments

Published on: February 23, 2024

1.4K
High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

33.3K

Area of Science:

  • Microbiology
  • Spectroscopy
  • Bioanalytical Chemistry

Background:

  • Fourier transform infrared (FT-IR) spectroscopy offers specific and reproducible signals for bacterial identification.
  • Current FT-IR methods for bacterial typing face challenges in accuracy due to variations in sample preparation and spectral acquisition.
  • Standardized protocols are needed to enhance the reliability of FT-IR for subspecies-level bacterial classification.

Purpose of the Study:

  • To present a unified and standardized protocol for bacterial typing using FT-IR spectroscopy.
  • To optimize sample preparation and spectral acquisition for improved FT-IR analysis.
  • To detail effective preprocessing and multivariate analysis techniques for spectral data.

Main Methods:

  • Development of a unified protocol encompassing bacterial culture, sample preparation, and FT-IR spectrum collection.
  • Implementation of standardized spectral acquisition parameters.
  • Application of specific preprocessing steps and multivariate statistical methods for data analysis.

Main Results:

  • The proposed protocol ensures specific and reproducible FT-IR signals for bacterial typing.
  • Standardized methods mitigate variability, enhancing typing accuracy at the subspecies level.
  • The protocol integrates sample handling, spectral acquisition, and data analysis for robust bacterial identification.

Conclusions:

  • A unified FT-IR protocol enhances the accuracy and reproducibility of bacterial typing.
  • Standardization of sample preparation and data analysis is crucial for reliable FT-IR based bacterial identification.
  • This protocol provides a robust framework for applying FT-IR spectroscopy in microbial diagnostics and research.