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Related Concept Videos

Transgenic Plants02:50

Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
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Rapid and highly efficient morphogenic gene-mediated hexaploid wheat transformation.

Kari Johnson1, Uyen Cao Chu1, Geny Anthony1

  • 1Corteva Agriscience, Johnston, IA, United States.

Frontiers in Plant Science
|April 17, 2023
PubMed
Summary

New wheat transformation methods use morphogenic genes to boost efficiency and speed. These simplified techniques reduce labor and costs, enabling faster generation of high-quality transgenic wheat lines.

Keywords:
Agrobacterium - mediated transformationTriticum aestivumWUSCHELmarker-free transformationmorphogenic genes

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Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Crop Science

Background:

  • Morphogenic regulator genes, such as Zm-Baby Boom (ZmBbm) and Zm-Wuschel2 (ZmWus2), have improved transformation in maize and sorghum by inducing somatic embryogenesis.
  • Efficient and cost-effective transformation methods are crucial for advancing wheat (Triticum aestivum L.) genetic improvement.

Purpose of the Study:

  • To develop and evaluate novel morphogenic gene-mediated wheat transformation methods.
  • To assess the efficiency, duration, and cost-effectiveness of these new transformation protocols.

Main Methods:

  • Two distinct wheat transformation protocols were developed utilizing morphogenic genes, with and without gene excision.
  • Methods involved Agrobacterium-mediated transformation and optimized tissue culture steps, eliminating embryonic axis excision and prolonged selection.
  • Transgenic event generation utilized herbicide (phosphinothricin, ethametsulfuron) and antibiotic (G418) selection across multiple wheat genotypes.

Main Results:

  • Transformation efficiencies of up to 58% and 75% were achieved with the developed methods.
  • Tissue culture duration was significantly reduced from 80 to approximately 50 days.
  • The simplified process reduced labor, increased throughput, and lowered costs compared to conventional methods.

Conclusions:

  • The reported morphogenic gene-mediated methods offer a highly efficient and simplified approach for wheat transformation.
  • These protocols facilitate the rapid generation of high-quality transgenic wheat events across diverse genotypes.
  • The advancements contribute to more cost-effective and high-throughput genetic engineering of wheat.