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Observation of the Ciliary Movement of Choroid Plexus Epithelial Cells Ex Vivo
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Phase-resolved dynamic wavefront imaging of cilia metachronal waves.
Yusi Miao1,2, Joseph C Jing1, Lidek Chou1
1Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA, USA.
Quantitative Imaging in Medicine and Surgery
|April 17, 2023
Summary
We developed a label-free imaging technique to visualize ciliary metachronal waves, enhancing diagnosis of respiratory diseases by revealing wave propagation and coordination for improved mucociliary clearance assessment.
Area of Science:
- Biomedical optics
- Respiratory physiology
- Microscopy
Background:
- Ciliary metachronal waves are crucial for mucociliary clearance (MCC) and immune response.
- Current diagnostic tools for assessing ciliary wave function are limited.
Purpose of the Study:
- To develop a label-free, high-speed imaging technique for visualizing ciliary metachronal waves.
- To quantitatively analyze spatial-temporal characteristics of ciliary wave motion.
Main Methods:
- Utilized spectrally-encoded interferometric microscopy for label-free, high-speed visualization (50 fps) of ciliary metachronal waves in tracheal explants.
- Applied phase-resolved enhanced dynamic (PHRED) analysis to time-series Doppler images for spatial-temporal characterization.
Main Results:
- PHRED analysis enabled distinguishing metachronal wave propagation direction and quantifying cilia beat amplitude and frequency.
- Phase-resolved dynamic wavefront imaging (PRDWI) distinctly visualized collective cilia movement direction and coordination compared to raw images.
Conclusions:
- The PRDWI technique offers a novel method for diagnosing respiratory diseases.
- This technique can evaluate treatment efficacy and advance biomedical research in respiratory health.
