Inspecting Histamine Isolated from Fish through a Highly Selective Molecularly Imprinted Electrochemical Sensor...

Area of Science:

• Analytical Chemistry
• Electrochemistry
• Materials Science

Background:

• Histamine accumulation in food poses health risks, with regulatory limits set by the FDA.
• Accurate and rapid detection of histamine is crucial for food safety and public health.

Purpose of the Study:

• To develop a novel electrochemical sensor for sensitive and selective histamine detection in fish products.
• To validate the performance of the developed sensor for real-world food analysis.

Main Methods:

• Fabrication of a molecularly imprinted polymer (MIP) film using electropolymerized polyurethane (PU) on a screen-printed electrode (SPE).
• Characterization of the MIP using Fourier-transform infrared (FTIR) spectroscopy.
• Electrochemical evaluation using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV).

Main Results:

• The MIP/SPE demonstrated a linear response for histamine detection from 1 to 1000 nmol L⁻¹.
• Achieved low detection limits of 1.765 nmol L⁻¹ for MIP/SPE and 709 nmol L⁻¹ for non-imprinted polymer (NIP)/SPE.
• The sensor successfully determined histamine levels in fish samples with accuracy and precision at room temperature.

Conclusions:

• The developed MIP-based electrochemical sensor offers a simple, low-cost, and effective method for histamine determination.
• This technique is suitable for on-site food safety monitoring, contributing to the prevention of histamine-induced toxicity.