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Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
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Slide-tags: scalable, single-nucleus barcoding for multi-modal spatial genomics
Biorxiv : the Preprint Server for Biology
|April 17, 2023
Summary
We developed Slide-tags, a novel method for spatial genomics that precisely maps individual cell gene expression within intact tissues. This technology enables high-resolution spatial transcriptomics and multiomic profiling across diverse human tissues.
Area of Science:
- Genomics
- Molecular Biology
- Bioinformatics
Background:
- High-throughput single-cell technologies have revolutionized tissue biology.
- Current methods lack routine spatial localization of profiled cells within complex tissues.
Conclusions:
- Slide-tags is a universal platform for spatial genomics, adaptable to various single-cell measurement technologies.
- Enables detailed spatial contextualization of cellular states and interactions in complex biological systems.
- Facilitates the integration of established single-cell data into the spatial genomics field.

