Related Experiment Video
Updated: Aug 2, 2025

Relating Stomatal Conductance to Leaf Functional Traits
Published on: October 12, 2015
Anatomical drivers of stomatal conductance in sorghum lines with different leaf widths grown under different
Yazen Al-Salman1,2, Francisco J Cano1,2,3, Ling Pan1,4,5
1ARC Centre of Excellence for Translational Photosynthesis, Canberra, ACT, Australia.
Abstract:
Sustaining crop productivity and resilience in water-limited environments and under rising temperatures are matters of concern worldwide. We investigated the leaf anatomical traits that underpin our recently identified link between leaf width (LW) and intrinsic water use efficiency (iWUE), as traits of interest in plant breeding. Ten sorghum lines with varying LW were grown under three temperatures to expand the range of variation of both LW and gas exchange rates. Leaf gas exchange, surface morphology and cross-sectional anatomy were measured and analysed using structural equations modelling. Narrower leaves had lower stomatal conductance (gs ) and higher iWUE across growth temperatures. They also had smaller intercellular airspaces, stomatal size, percentage of open stomatal aperture relative to maximum, hydraulic pathway, mesophyll thickness, and leaf mass per area. Structural modelling revealed a developmental association among leaf anatomical traits that underpinned gs variation in sorghum. Growing temperature and LW both impacted leaf gas exchange rates, but only LW directly impacted leaf anatomy. Wider leaves may be more productive under well-watered conditions, but consume more water for growth and development, which is detrimental under water stress.
More Related Videos
12:11Measurement of Leaf Hydraulic Conductance and Stomatal Conductance and Their Responses to Irradiance and Dehydration Using the Evaporative Flux Method EFM
Published on: December 31, 2012
07:08Identification of Novel Regulators of Plant Transpiration by Large-Scale Thermal Imaging Screening in Helianthus Annuus
Published on: January 30, 2020
Related Concept Videos
Regulation of Transpiration by Stomata
Responses to Heat and Cold Stress
Adaptations that Reduce Water Loss
Responses to Drought and Flooding
Responses to Salt Stress
Light Acquisition