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Updated: Aug 2, 2025

Muscle Imbalances: Testing and Training Functional Eccentric Hamstring Strength in Athletic Populations
Published on: May 1, 2018
A Comparison of Squat Depth and Sex on Knee Kinematics and Muscle Activation
Kiara B Barrett1, Zachary A Sievert1, Hunter J Bennett1
1Neuromechanics Lab, Department of Human Movement Sciences, Old Dominion University, Norfolk, VA 23526.
Abstract:
The squat is an essential exercise for strengthening lower body musculature. Although squats are frequently employed to improve lower extremity strength and neuromuscular control, differences between sexes and slight modifications, such as squat depth, can dramatically alter muscle recruitment and thus the foci of the exercise. The purpose of this study was to assess the effect of sex and squat depth on lower extremity coactivation and kinematics. Twenty recreationally active (female = 10) participants were recruited. The first visit consisted of one repetition maximum testing. For the second visit, muscle activation was recorded of the gluteus maximus (GM), semitendinosus, biceps femoris (BF), vastus medialis, vastus lateralis, rectus femoris, and gastrocnemius. Reflective markers were placed on the lower body for three-dimensional motion capture. Participants performed a series of squats to 90 deg knee flexion and 120 deg knee flexion. Benjamin-Hochberg procedure was employed and the alpha level was set at 0.05. Knee flexion (p < 0.001), adduction (p < 0.001), and external rotation (p = 0.008) were reduced during 90 deg compared to deep squats. Hip flexion, abduction, and external rotation were greater in deep squats (p < 0.001). Males had greater hip extensor to quad (HE:Q) cocontraction in 90 deg compared to deep squats (p = 0.007); females produced greater posterior chain activation in deep squats (p = 0.001) on ascent. When comparing sexes, males displayed greater HE:Q in the 90 deg squat during ascent (p = 0.013). The addition of deep squats into a preventative training program could be beneficial in reducing deficits prevalent in females and decrease injury incidence.
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