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Updated: Aug 2, 2025

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Published on: June 1, 2011
Reagentless protein-based electrochemical biosensors
Saimon M Silva1,2,3,4, Miaosi Li1, Alexandre Xavier Mendes5,2
1Universal Biosensors, Rowville, Victoria 3178, Australia. smoraessilva@swin.edu.au.
Abstract:
The creation of reagentless protein-based biosensors that are capable of monitoring molecular analytes directly in bodily fluids could revolutionize our understanding of biology and personalized health monitoring. The limited number of molecular sensors that are currently available in the market depends on the specific enzymatic or chemical reactivity of their target analytes and therefore are not applicable to many relevant biomarkers. Aiming to overcome this limited molecular sensing generality, a new class of reagentless protein-based electrochemical sensors has been introduced for the direct measurements of biomarkers in unprocessed biological fluids. This mini-review will discuss the most recent cutting-edge discoveries for the development of electroanalytical modular biosensors, where all the sensors' components are integrated into a self-sufficient sensor allowing hence its autonomous functionality.
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