Lipid peroxidation in diamond supported bilayers
A R Ortiz Moreno1, R Li1, K Wu1
1Groningen University, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AW Groningen, the Netherlands. romana.schirhagl@gmail.com.
Nanoscale
|April 17, 2023
Summary
This study introduces a diamond quantum sensing technique for observing short-lived radical intermediates during lipid peroxidation. This method enables nanoscale imaging of these damaging molecules within artificial cell membranes.
Area of Science:
- Biochemistry
- Materials Science
- Quantum Sensing
Background:
- Lipid peroxidation, triggered by oxidative stress, generates toxic and carcinogenic byproducts.
- Understanding lipid peroxidation at the nanoscale is crucial due to its damaging effects on cell membranes.
- Short-lived radical intermediates are key drivers of lipid peroxidation but are challenging to measure.
Purpose of the Study:
- To investigate lipid peroxidation in artificial lipid bilayers using a novel diamond-based sensing approach.
- To develop and demonstrate a method for *in situ* nanoscale imaging of lipid peroxidation radical intermediates.
Main Methods:
- Utilized artificial lipid bilayers immobilized on a diamond substrate.
- Employed a diamond quantum sensing technique known as *T*1-relaxometry.
- Performed *in situ* measurements and imaging of radical intermediates during lipid peroxidation.
Main Results:
- Successfully demonstrated the capability of *T*1-relaxometry for studying lipid peroxidation.
- Achieved nanoscale resolution in imaging radical intermediates within artificial lipid membranes.
- Provided insights into the behavior of reactive radical intermediates during the peroxidation process.
Conclusions:
- Diamond quantum sensing, specifically *T*1-relaxometry, is a powerful tool for studying lipid peroxidation dynamics.
- The developed method allows for unprecedented *in situ* nanoscale observation of critical radical intermediates.
- This approach opens new avenues for understanding oxidative stress and membrane damage at the molecular level.
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