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Lipid peroxidation in diamond supported bilayers.

A R Ortiz Moreno1, R Li1, K Wu1

  • 1Groningen University, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AW Groningen, the Netherlands. romana.schirhagl@gmail.com.

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Summary

This study introduces a diamond quantum sensing technique for observing short-lived radical intermediates during lipid peroxidation. This method enables nanoscale imaging of these damaging molecules within artificial cell membranes.

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Area of Science:

  • Biochemistry
  • Materials Science
  • Quantum Sensing

Background:

  • Lipid peroxidation, triggered by oxidative stress, generates toxic and carcinogenic byproducts.
  • Understanding lipid peroxidation at the nanoscale is crucial due to its damaging effects on cell membranes.
  • Short-lived radical intermediates are key drivers of lipid peroxidation but are challenging to measure.

Purpose of the Study:

  • To investigate lipid peroxidation in artificial lipid bilayers using a novel diamond-based sensing approach.
  • To develop and demonstrate a method for *in situ* nanoscale imaging of lipid peroxidation radical intermediates.

Main Methods:

  • Utilized artificial lipid bilayers immobilized on a diamond substrate.
  • Employed a diamond quantum sensing technique known as *T*1-relaxometry.
  • Performed *in situ* measurements and imaging of radical intermediates during lipid peroxidation.

Main Results:

  • Successfully demonstrated the capability of *T*1-relaxometry for studying lipid peroxidation.
  • Achieved nanoscale resolution in imaging radical intermediates within artificial lipid membranes.
  • Provided insights into the behavior of reactive radical intermediates during the peroxidation process.

Conclusions:

  • Diamond quantum sensing, specifically *T*1-relaxometry, is a powerful tool for studying lipid peroxidation dynamics.
  • The developed method allows for unprecedented *in situ* nanoscale observation of critical radical intermediates.
  • This approach opens new avenues for understanding oxidative stress and membrane damage at the molecular level.