Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of Diabetes Mellitus on Drug-Induced Liver Injury during Antituberculosis Therapy: A Prospective Cohort Study.

The American journal of tropical medicine and hygiene·2026
Same author

Intercellular chemerin-cmklr1 couples epicardial mechanosensing to fibroblasts in pressure overload.

Nature communications·2026
Same author

A Bone Marrow-Mimetic Hydrogel Enables Dual-Phase Hemostasis and Vascularized Osteogenesis for Cranial Defects.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Geographic and population disparities in cutaneous melanoma in the United States: state-level trends and national population-level analyses.

BMC public health·2026
Same author

A cardiac fibrosis specific circRNA_006640 sponges miR-7648-3p and miR-185-3p to synergistically up-regulate CTGF.

Molecular and cellular biochemistry·2026
Same author

A Nano-Interception Strategy for Chronic Heart Failure: Prussian Blue Nanoparticles Disrupt Fibroblast-Immune Communication via CCL2 Sequestration.

Advanced materials (Deerfield Beach, Fla.)·2026

Related Experiment Video

Updated: Aug 2, 2025

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning
12:07

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Published on: April 16, 2018

13.5K

Bilayer Hydrogels by Reactive-Induced Macrophase Separation.

Dong Zhang1, Yijing Tang1, Xiaomin He2

  • 1Department of Chemical, Biomolecular, and Corrosion Engineering, The University of Akron, Akron, Ohio 44325, United States.

ACS Macro Letters
|April 17, 2023
PubMed
Summary

A new one-pot strategy simplifies creating bilayer hydrogels with seamless interfaces. This advance enables diverse applications in engineered tissues and human-machine interfaces using smart materials.

More Related Videos

Synthesis of PolyN-isopropylacrylamide Janus Microhydrogels for Anisotropic Thermo-responsiveness and Organophilic/Hydrophilic Loading Capability
09:09

Synthesis of PolyN-isopropylacrylamide Janus Microhydrogels for Anisotropic Thermo-responsiveness and Organophilic/Hydrophilic Loading Capability

Published on: February 27, 2016

10.1K
Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
10:45

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Published on: September 29, 2016

13.1K

Related Experiment Videos

Last Updated: Aug 2, 2025

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning
12:07

Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Published on: April 16, 2018

13.5K
Synthesis of PolyN-isopropylacrylamide Janus Microhydrogels for Anisotropic Thermo-responsiveness and Organophilic/Hydrophilic Loading Capability
09:09

Synthesis of PolyN-isopropylacrylamide Janus Microhydrogels for Anisotropic Thermo-responsiveness and Organophilic/Hydrophilic Loading Capability

Published on: February 27, 2016

10.1K
Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
10:45

Light-mediated Formation and Patterning of Hydrogels for Cell Culture Applications

Published on: September 29, 2016

13.1K

Area of Science:

  • Materials Science
  • Polymer Chemistry
  • Biomaterials Engineering

Background:

  • Bilayer hydrogels offer tunable properties for advanced applications like engineered tissues and human-machine interfaces.
  • Current fabrication methods for anisotropic bilayer hydrogels are complex, multistep, and result in poor interfacial adhesion, limiting their utility.
  • There is a need for simplified, versatile strategies to produce high-quality bilayer hydrogels.

Purpose of the Study:

  • To develop a general, one-pot strategy for fabricating bilayer hydrogels with seamless interfaces.
  • To demonstrate control over layer separation efficiency in the fabricated hydrogels.
  • To expand the range of accessible bilayer hydrogel compositions using diverse monomers.

Main Methods:

  • Employed a macrophase separation strategy utilizing competitive polymerization between vinyl and styryl monomers.
  • Decoupled two distinct gelation processes to form vinyl- and styryl-enriched layers within a single pot.
  • Manipulated reaction conditions to achieve controllable layer separation efficiencies ranging from 20% to 99%.

Main Results:

  • Successfully fabricated a family of bilayer hydrogels with seamless interfaces using a one-pot method.
  • Achieved precise control over the layer separation efficiency, demonstrating the strategy's versatility.
  • Showcased the potential for using a wide array of radical monomers beyond currently available options.

Conclusions:

  • The reported macrophase separation strategy provides a straightforward and efficient approach to bilayer hydrogel fabrication.
  • This method overcomes limitations of conventional techniques, offering improved interfacial integrity and design flexibility.
  • The developed technique facilitates the creation of next-generation bilayer hydrogels for diverse scientific and technological applications.