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Updated: Aug 2, 2025
Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
Published on: May 13, 2019
Structures of human primosome elongation complexes
Qixiang He1, Andrey G Baranovskiy2, Lucia M Morstadt2
1Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA.
Abstract:
The synthesis of RNA-DNA primer by primosome requires coordination between primase and DNA polymerase α subunits, which is accompanied by unknown architectural rearrangements of multiple domains. Using cryogenic electron microscopy, we solved a 3.6 Å human primosome structure caught at an early stage of RNA primer elongation with deoxynucleotides. The structure confirms a long-standing role of primase large subunit and reveals new insights into how primosome is limited to synthesizing short RNA-DNA primers.
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