T-RHEX-RNAseq - a tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded
Charlotte Gustafsson1, Julia Hauenstein1, Nicolai Frengen1
1Department of Laboratory Medicine, Division of Clinical Immunology, Karolinska Institutet, ANA Futura, Alfred Nobels Allé 8 floor 7, Huddinge, SE-141 52, Sweden.
Background:
RNA sequencing has become the mainstay for studies of gene expression. Still, analysis of rare cells with random hexamer priming - to allow analysis of a broader range of transcripts - remains challenging.
Results:
We here describe a tagmentation-based, rRNA blocked, random hexamer primed RNAseq approach (T-RHEX-RNAseq) for generating stranded RNAseq libraries from very low numbers of FACS sorted cells without RNA purification steps.
Conclusion:
T-RHEX-RNAseq provides an easy-to-use, time efficient and automation compatible method for generating stranded RNAseq libraries from rare cells.
