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Isolation of new Klebsiella pneumoniae phage PSKP16

Sara Sadeqi1, Slawomir Michniewski2, Farhad Nikkhahi3

  • 1Department of Medical Microbiology, Qazvin University of Medical Sciences, Qazvin, Iran.

Iranian Journal of Microbiology
|April 18, 2023
PubMed
Abstract

Insights

A novel lytic bacteriophage, PSKP16, was identified for treating Klebsiella pneumoniae infections. This phage offers a promising avenue for combating antimicrobial resistance, a critical global health challenge.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Klebsiella pneumoniae is a major cause of nosocomial infections and antimicrobial resistance deaths.
  • Phage therapy presents a viable alternative for treating infections caused by drug-resistant bacteria.

Purpose of the Study:

  • To isolate and characterize a bacteriophage effective against Klebsiella pneumoniae.
  • To evaluate the potential of phage therapy in combating antibiotic-resistant Klebsiella pneumoniae.

Main Methods:

  • Isolation of bacteriophage PSKP16 against Klebsiella pneumoniae (capsular type K2).
  • Morphological and genomic characterization of the isolated phage, including genome size and GC content.
  • Phylogenetic analysis to determine evolutionary proximity to other Klebsiella phages.

Main Results:

  • Phage PSKP16, a lytic phage with Siphovirus-like morphology, was successfully isolated.
  • PSKP16 possesses a linear double-stranded DNA genome of 46,712 bp with 67 predicted ORFs.
  • The phage belongs to the Webervirus genus and is closely related to other Klebsiella phages.

Conclusions:

  • Phage isolation is an efficient method for discovering novel therapeutic agents.
  • Thorough characterization of isolated phages is crucial for ensuring safety in phage therapy applications.
  • PSKP16 represents a potential candidate for phage therapy against Klebsiella pneumoniae infections.
Keywords:
BacteriophagesDrug resistanceHigh throughput nucleotide sequencingInfection controlKlebsiella pneumoniae

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