Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

13.9K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
13.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stepwise mitochondria-targeted photodynamic immunotherapy enabled by an outer membrane vesicles-based nanoplatform for synergistic tumor ablation and immune reprogramming.

Smart molecules : open access·2026
Same author

Multicolor and Attenuated Light Intensity Responses in Protein Hydrogels Arising from Photoregulated Crosslink Dynamics.

Angewandte Chemie (International ed. in English)·2026
Same author

Let There be Light! Light as an Engine and Regulator in Synthetic Cells.

Angewandte Chemie (International ed. in English)·2026
Same author

Autonomous Cargo Transport with Biohybrid Microswimmers Enabled by Light-Mediated Bacteria-Cargo Communication.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Assembling Lipid Membrane Scaffolds on Microgel-Based Artificial Cells through Vesicle Fusion onto the Hydrogel Network.

ACS nano·2026
Same author

Scube2 primes Dispatched and ADAM10-mediated Shh release by recruiting HDL acceptors to the plasma membrane.

Communications biology·2026

Related Experiment Video

Updated: Aug 2, 2025

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor
06:03

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor

Published on: March 22, 2024

979

An Adenosylcobalamin Specific Whole-Cell Biosensor.

Juan José Quispe Haro1, Seraphine V Wegner1

  • 1Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Waldeyerstrasse 15, 48149, Münster, Germany.

Advanced Healthcare Materials
|April 18, 2023
PubMed
Summary

A novel whole-cell biosensor specifically detects adenosylcobalamin (AdoB12), a vital form of vitamin B12. This agglutination assay offers a simple, point-of-care method for monitoring AdoB12 levels, crucial for preventing deficiency-related health issues.

Keywords:
CarHadenosylcobalaminagglutination assaysbiosensorsvitamin B12

More Related Videos

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

9.1K
A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
10:12

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

Published on: August 8, 2013

16.8K

Related Experiment Videos

Last Updated: Aug 2, 2025

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor
06:03

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor

Published on: March 22, 2024

979
The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
08:06

The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

Published on: February 1, 2018

9.1K
A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
10:12

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

Published on: August 8, 2013

16.8K

Area of Science:

  • Biotechnology
  • Biosensor Development
  • Analytical Chemistry

Background:

  • Vitamin B12 (cobalamin) is crucial for human health, with deficiency causing anemia and neurological issues.
  • Existing sensors struggle to differentiate between various bioactive forms of vitamin B12.
  • Adenosylcobalamin (AdoB12) is a key bioactive form requiring specific detection methods.

Purpose of the Study:

  • To develop a whole-cell biosensor for specific detection of adenosylcobalamin (AdoB12).
  • To create a simple, point-of-care assay for monitoring AdoB12 levels, particularly in high-dose supplementation scenarios.

Main Methods:

  • Engineered Escherichia coli to express the AdoB12-specific binding domain of CarH on their surface.
  • Utilized CarH tetramerization-induced bacterial agglutination as the detection signal.
  • Employed green light illumination to trigger tetramer disassembly and serve as an internal quality control.

Main Results:

  • The biosensor demonstrated specific agglutination in the presence of AdoB12.
  • Achieved a detection limit of 500 nM AdoB12 in protein-poor biofluids like urine.
  • Showcased high specificity for AdoB12 over other vitamin B12 forms, validated with supplements.

Conclusions:

  • A proof-of-concept for a cost-effective, easy-to-read AdoB12 sensor was established.
  • The developed biosensor is suitable for point-of-care applications.
  • This technology can aid in monitoring high-dose vitamin B12 supplementation and managing deficiency.