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Updated: Aug 2, 2025
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Innervation of Human Intestinal Organoids
Published on: January 17, 2025
580
Transplanted human intestinal organoids: a resource for modeling human intestinal development
Akaljot Singh1, Holly M Poling1, Praneet Chaturvedi2,3
1Division of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA.
Development (Cambridge, England)
|April 18, 2023
Summary
Human intestinal organoids (HIOs) transplanted into animal models recapitulate fetal intestinal development. This study details HIO maturation stages, confirming their value for studying early human gut development.
Area of Science:
- Developmental Biology
- Stem Cell Biology
- Gastroenterology
Background:
- Human intestinal organoids (HIOs) are complex 3D structures derived from pluripotent stem cells.
- HIOs can form laminated structures resembling native human intestine upon transplantation into animal hosts.
- While HIO engraftment endpoints are known, their developmental trajectory remains underexplored.
Purpose of the Study:
- To elucidate the developmental stages of HIO engraftment after transplantation.
- To determine if HIO engraftment parallels fetal human intestinal development.
- To validate HIOs as a model for early human intestinal development.
Main Methods:
- Histological analysis of transplanted HIOs at 2, 4, 6, and 8 weeks post-transplantation.
- Single-nuclear RNA sequencing to track cell population emergence over time.
- In situ protein expression analysis to validate transcriptomic findings.
Main Results:
- HIO maturation closely mirrors key stages of fetal human intestinal development.
- Distinct cell populations emerge over time in a manner consistent with early development.
- Transcriptomic data was validated by corresponding protein expression patterns.
Conclusions:
- Transplanted HIOs recapitulate early human intestinal development.
- HIOs serve as a valuable in vivo model system for studying human intestinal development.
- This study provides a detailed temporal map of HIO engraftment and maturation.
