Revisiting the mark test for mirror self-recognition

Despite half a century of research, few species have exhibited convincing evidence of mirror self-recognition. Several methodological objections to Gallup's mark test have been raised, but empirical studies have shown that methodology does not adequately explain why most species fail to recognize themselves in mirrors. However, a potential issue was constantly overlooked: mirror ecological relevance. Although reflective surfaces in nature are horizontal, previous studies indeed used vertical mirrors. The present study revisited the mark test to address this issue in an experiment with capuchin monkeys (Sapajus apella). Additionally, a new procedure based on sticker exchange was designed to maximize mark attractiveness. Subjects were first trained to exchange stickers, habituated to being head-touched, and exposed to a horizontal mirror. Then, their mirror self-recognition was tested by surreptitiously placing a sticker on their forehead before requesting them to exchange stickers. None of the monkeys removed the sticker from their forehead in the presence of the mirror. In line with previous studies, this result suggests that capuchin monkeys lack the ability to recognize themselves in mirrors. Nonetheless, this modified mark test could prove useful in future studies, including investigation of interindividual variability of mirror self-recognition in self-recognizing species.