The effects of intrinsic water-use efficiency and climate on wood anatomy

Climate warming may induce growth decline in warm-temperate areas subjected to seasonal soil moisture deficit, whereas increasing atmospheric CO₂ concentration (C_a) is expected to enhance tree growth. An accurate understanding of tree growth and physiological processes responding to climate warming and increasing C_a is critical. Here, we analyzed tree-ring stable carbon isotope and wood anatomical traits of Pinus tabuliformis from Qinling Mountains in China to understand how lumen diameter (LD) determining potential hydraulic conductivity and cell-wall thickness (CWT) determining carbon storage responded to climate and C_a. The effects of climate and C_a on intrinsic water-use efficiency (iWUE) were isolated, and iWUE values due to only-climate (iWUE_Clim) and only-CO₂ effects (iWUE_CO2) were obtained. During a low-iWUE period, the influences of climate on earlywood (EW) LD and latewood (LW) CWT prevailed. During a high-iWUE period, CO₂ fertilization promoted cell enlargement and carbon storage but this was counteracted by a negative influence of climate warming. The limiting direct effects of iWUE_Clim and indirect effects of climate on EW LD were greater than on LW CWT. P. tabuliformis in temperate forests will face a decline of growth and carbon fixation, but will produce embolism-resistant tracheids with narrow lumen responding to future hotter droughts.