Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

5.7K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.7K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
MicroRNAs01:22

MicroRNAs

3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A coordinated transcriptional program controls de novo Golgi biogenesis.

The EMBO journal·2026
Same author

Deletion of endothelial SOX9 reverses aging-induced cardiac dysfunction.

Cardiovascular research·2026
Same author

The Floor-Ceiling-Chip, or 2 × 2D = Pseudo-3D-Approaching 3D Cell Morphology and Organization between Two Opposing 2D Substrates with Cell-Adhesive Protein Micropatterns.

Advanced healthcare materials·2026
Same author

Circulating Musclin is associated with skeletal muscle function and subclinical cardiac dysfunction in patients with cancer.

British journal of pharmacology·2026
Same author

Immunomodulatory topographies regulate myofibroblast differentiation and influence fibrous encapsulation of glaucoma drainage devices.

Bioactive materials·2026
Same author

Controlling spatial organization of the cell - using surface micro-pillar patterns to manipulate Golgi morphology and function.

Journal of cell science·2026
Same journal

Neonatal systemic gene therapy restores cardiorespiratory function in a rat model of Pompe disease.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

Endothelial injury with capillary leak: A final common pathway in acute AAV toxicity?

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

Long-term functional synaptic integration of genome-edited retinal organoids in a primate model of macular degeneration.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

Nox1/4 inhibitor Setanaxib treatment ameliorates cardiac function in mouse models of Duchenne Muscular Dystrophy.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

HSP90α-USP7-DNMT1 Axis Drives Hepatocellular Carcinoma Recurrence After Microwave Ablation by Disrupting ACSS3-Mediated Propionate Metabolism.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same journal

Cell-penetrating asymmetric siRNA targeting MyD88 suppresses inflammasome-driven ocular degeneration and angiogenesis.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
See all related articles

Related Experiment Video

Updated: Aug 2, 2025

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

7.5K

MicroRNA-216a is essential for cardiac angiogenesis.

Rio P Juni1, Jordy M M Kocken2, Ricardo C Abreu3

  • 1CARIM School for Cardiovascular Diseases, Faculty of Health, Medicine and Life Sciences, Maastricht University, 6229 ER Maastricht, the Netherlands; Department of Physiology, Amsterdam University Medical Centers, De Boelelaan 1117, 1081 HV Amsterdam, the Netherlands.

Molecular Therapy : the Journal of the American Society of Gene Therapy
|April 19, 2023
PubMed
Summary
This summary is machine-generated.

MicroRNAs regulate blood vessel growth in the heart. Reduced miR-216a in cardiac stress impairs vascularization, leading to heart failure phenotypes in mice.

Keywords:
angiogenesisautophagycardiac remodelingendothelial cellsheart failuremicroRNAs

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.2K

Related Experiment Videos

Last Updated: Aug 2, 2025

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

7.5K
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.2K

Area of Science:

  • Cardiovascular Biology
  • Molecular Biology
  • Genetics

Background:

  • Impaired myocardial vascularization causes oxygen supply-demand mismatch in heart failure.
  • MicroRNAs (miRNAs) are implicated in angiogenesis, but their role in heart failure is unclear.
  • Endothelial cell proliferation is a key parameter in angiogenesis.

Purpose of the Study:

  • To identify microRNAs regulating endothelial cell proliferation.
  • To investigate the role of miR-216a in cardiac vascularization and heart failure.

Main Methods:

  • High-throughput functional screening of a whole-genome miRNA silencing library.
  • Phenotypic analysis of endothelial cell proliferation.
  • Generation and analysis of miR-216a null mice.

Main Results:

  • Several pro- and anti-angiogenic miRNAs were identified.
  • miR-216a was identified as a pro-angiogenic miRNA enriched in cardiac microvascular endothelial cells.
  • miR-216a null mice exhibited impaired cardiac vascularization, autophagy, and inflammation.

Conclusions:

  • miR-216a is crucial for maintaining cardiac microvascularization.
  • Reduced miR-216a contributes to cardiac dysfunction under stress.
  • miRNA regulation of microvascularization is vital for the cardiac response to stress.