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Updated: Jul 3, 2026
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
Electrochemical biosensor based on antibody-modified Au nanoparticles for rapid and sensitive analysis of influenza A
Qiwen Bao1, Gang Li1, Zhengchun Yang2
1School of Precision Instrument and Optoelectronic Engineering, the State Key Laboratory of Precision Measuring Technology and Instruments, Tianjin University, 92 Weijin Road, Tianjin, 300072 China.
Abstract:
To cope with the easy transmissibility of the avian influenza A virus subtype H1N1, a biosensor was developed for rapid and highly sensitive electrochemical immunoassay. Based on the principle of specific binding between antibody and virus molecules, the active molecule-antibody-adapter structure was formed on the surface of an Au NP substrate electrode; it included a highly specific surface area and good electrochemical activity for selective amplification detection of the H1N1 virus. The electrochemical test results showed that the BSA/H1N1 Ab/Glu/Cys/Au NPs/CP electrode was used for the electrochemical detection of the H1N1 virus with a sensitivity of 92.1 µA (pg/mL)-1 cm2, LOD of 0.25 pg/ml, linear ranges of 0.25-5 pg/mL, and linearity of (R 2 = 0.9846). A convenient H1N1 antibody-based electrochemical electrode for the molecular detection of the H1N1 virus will be of great use in the field of epidemic prevention and raw poultry protection.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s11581-023-04944-w.
