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Interplay between NIN-LIKE PROTEINs 6 and 7 in nitrate signaling
Yu-Hsuan Cheng1,2, Mickael Durand3, Virginie Brehaut3
1Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and National Defense Medical Center, Taipei 114201, Taiwan.
Plant Physiology
|April 19, 2023
Summary
NIN-LIKE-PROTEIN 6 (NLP6) and NIN-LIKE-PROTEIN 7 (NLP7) are crucial for plant nitrate response. They exhibit distinct and redundant roles, particularly under high nitrate and ammonium conditions, influencing gene expression and growth.
Area of Science:
- Plant molecular biology
- Nitrogen signaling pathways
- Transcriptional regulation
Background:
- NIN-LIKE-PROTEIN 7 (NLP7) is the primary regulator of the plant primary nitrate response (PNR).
- The function of its homolog, NIN-LIKE-PROTEIN 6 (NLP6), in nitrogen signaling and its interaction with NLP7 are not fully understood.
Purpose of the Study:
- To elucidate the role of NLP6 in nitrogen signaling and its interplay with NLP7.
- To investigate the distinct and overlapping functions of NLP6 and NLP7 in response to different nitrogen sources and concentrations.
Main Methods:
- Analysis of nuclear localization and nucleocytosolic shuttling of NLP6 and NLP7.
- Phenotypic analysis of single and double nlp6nlp7 mutants under varying nitrate conditions.
- Transcriptome analysis to identify genes regulated by NLP6 and NLP7.
- Comparative analysis of NLP family member roles in PNR.
Main Results:
- Nuclear localization of NLP6 is nitrate-dependent, similar to NLP7, but their shuttling is independent.
- The nlp6nlp7 double mutant shows synergistic growth retardation under nitrate stress.
- NLP6 and NLP7 regulate approximately 50% of nitrate-induced genes, with distinct roles in different gene clusters.
- NLP6 and NLP7 are functionally redundant and act as repressors under high ammonium conditions.
- NLP6 and NLP7 play a more significant role in response to high nitrate concentrations.
Conclusions:
- NLP6 and NLP7 exhibit complex interplay, with both distinct and partially redundant functions depending on the nitrogen source and specific gene clusters.
- These proteins play critical roles in both nitrate and ammonium signaling pathways.
- Multiple NLP family members contribute to the PNR through diverse regulatory mechanisms.
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