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Related Experiment Videos

Ag(c): recognition by a monoclonal antibody.

M T Robinson, V N Schumaker, R Butler

    Arteriosclerosis (Dallas, Tex.)
    |May 1, 1986
    PubMed
    Summary

    Researchers identified a specific factor (Ag(c)) on apolipoprotein B, linking it to chromosome 2. This finding advances understanding of genetic variations in apolipoprotein B and potential links to atherosclerosis.

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    Molecular genetics of familial hypercholesterolaemia in Norway.

    Journal of internal medicine·1997

    Area of Science:

    • Genetics
    • Immunology
    • Biochemistry

    Background:

    • Apolipoprotein B (apoB) is a key component of low-density lipoprotein (LDL).
    • Allelic variations in apoB have been previously detected using specific antibodies.
    • The Ag system refers to genetic variations in apoB.

    Purpose of the Study:

    • To identify the Ag(c) factor with a specific monoclonal antibody against human apolipoprotein B.
    • To map the Ag system to the structural gene for apoB and its chromosomal location.
    • To investigate the relationship between apoB allelic variations and atherosclerosis.

    Main Methods:

    • Utilized a monoclonal antibody targeting human apolipoprotein B.
    • Performed family studies to detect allelic variations.
    • Identified the Ag(c) factor and mapped its epitope.
    • Correlated findings with LDL composition and animal studies on atherosclerosis.

    Main Results:

    • The monoclonal antibody was identified with the Ag(c) factor.
    • The Ag system was localized to the structural gene for apolipoprotein B on chromosome 2.
    • The Ag(c) epitope is present in a common amino acid sequence of apoB-100 and apoB-48.
    • Individual LDL particles exhibit either the Ag(c) or Ag(g) epitope.

    Conclusions:

    • The Ag(c) factor's identification refines the mapping of the Ag system to human chromosome 2.
    • Understanding apoB allelic variations, like Ag(c)/Ag(g), is crucial for studying LDL structure and function.
    • These findings provide a basis for further research into the genetic underpinnings of atherosclerosis.

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