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Updated: Jul 25, 2026

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
Published on: May 22, 2014
Identifying Inflammasome Activation in Human Tissues
Eleonora Mezzaroma1,2, Adolfo Mauro1,3, Stefano Toldo4
1VCU Health Pauley Heart Center, Virginia Commonwealth University, Richmond, VA, USA.
Abstract:
The NACHT, LRR, and PYD domain-containing protein-3 (NLRP3) inflammasome activation is part of a stereotyped cellular response to injury or infection. The NLRP3 inflammasome activation promotes cellular dysfunction and death, leading to local and systemic inflammation, organ dysfunction, and adverse outcome. Immunohistochemistry and immunofluorescence can be used to determine whether the NLRP3 inflammasome components are present in human biopsy or autopsy tissue samples.

