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Identifying Inflammasome Activation in Human Tissues.

Eleonora Mezzaroma1,2, Adolfo Mauro1,3, Stefano Toldo4

  • 1VCU Health Pauley Heart Center, Virginia Commonwealth University, Richmond, VA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 19, 2023
PubMed
Summary

The NACHT, LRR, and PYD domain-containing protein-3 (NLRP3) inflammasome activation contributes to cell damage and inflammation. Detecting NLRP3 inflammasome components in tissues can help assess its role in disease.

Keywords:
ASCCaspase-1HeartHuman tissuesImmunofluorescenceImmunohistochemistryInflammasomeLungNLRP3

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Area of Science:

  • Immunology
  • Cellular Biology
  • Pathology

Background:

  • NLRP3 inflammasome activation is a cellular response to injury or infection.
  • This activation leads to cellular dysfunction, death, and inflammation.
  • It can result in organ dysfunction and adverse outcomes.

Purpose of the Study:

  • To highlight the role of NLRP3 inflammasome activation in cellular dysfunction and inflammation.
  • To introduce methods for detecting NLRP3 inflammasome components in human tissues.

Main Methods:

  • Immunohistochemistry
  • Immunofluorescence
  • Analysis of human biopsy and autopsy tissue samples

Main Results:

  • NLRP3 inflammasome components can be detected in human tissue samples.
  • The presence of these components indicates inflammasome activation.

Conclusions:

  • NLRP3 inflammasome activation is a significant factor in cellular damage and inflammation.
  • Immunohistochemistry and immunofluorescence are valuable tools for studying NLRP3 inflammasome in clinical pathology.