Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Studying the Cytoskeleton01:17

Studying the Cytoskeleton

6.3K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
6.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Common cervicovaginal sequencing methods result in discordant molecular diagnoses of bacterial vaginosis and reveal strain level effects of <i>Gardnerella vaginalis</i>.

npj women's health·2026
Same author

Intranasal replicon vaccine establishes mucosal immunity and protects against H5N1 and H7N9 influenza.

Nature communications·2026
Same author

Distinct gastrointestinal microbial signatures predict parasite levels in controlled Plasmodium infections in both rhesus macaques and humans.

Nature communications·2025
Same author

Analysis of SIV Spatiotemporal Dissemination Patterns in Rhesus Macaques During Early Rectal Transmission Demonstrates Systemic Infection Does Not Require Viral Local Amplification at the Entry Portal.

bioRxiv : the preprint server for biology·2025
Same author

Host-microbe interactions characterized by gene expression of cervical adhesion molecules, cytokines, and growth factors define the recurrence of bacterial vaginosis.

Research square·2025
Same author

Elevated peripheral and nervous system inflammation is associated with decreased short-chain fatty acid levels in Zika virus-infected macaques.

Journal of virology·2025

Related Experiment Video

Updated: Aug 2, 2025

Author Spotlight: Flow Cytometric Determination of Pyroptosis in Avian Cells
05:14

Author Spotlight: Flow Cytometric Determination of Pyroptosis in Avian Cells

Published on: May 31, 2024

1.3K

Assessing Cytoskeletal Destruction During Pyroptosis.

Michael A Davis1, Michael Gale2

  • 1Center for Innate Immunity and Immune Disease, Department of Immunology, University of Washington, Seattle, WA, USA. madphd@uw.edu.

Methods in Molecular Biology (Clifton, N.J.)
|April 19, 2023
PubMed
Summary
This summary is machine-generated.

Pyroptosis, an inflammatory cell death, involves gasdermin-D but not colloid-osmotic lysis. Cytoskeletal disruptions, not swelling, cause cell rupture, requiring new detection methods.

Keywords:
ActinCytoskeletonIntermediate filamentsLaminMicrotubulesNuclear laminaPyroptosisTubulinVimentin

More Related Videos

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
06:52

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

10.8K
Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.3K

Related Experiment Videos

Last Updated: Aug 2, 2025

Author Spotlight: Flow Cytometric Determination of Pyroptosis in Avian Cells
05:14

Author Spotlight: Flow Cytometric Determination of Pyroptosis in Avian Cells

Published on: May 31, 2024

1.3K
Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
06:52

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

10.8K
Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.3K

Area of Science:

  • Cell Biology
  • Immunology
  • Biochemistry

Background:

  • Pyroptosis is an inflammatory cell death pathway mediated by caspase-1/caspase-11 and gasdermin-D.
  • Previously, pyroptosis was characterized by colloid-osmotic lysis, but recent findings suggest cells do not swell.
  • Calpain-mediated vimentin cleavage leads to intermediate filament loss, rendering cells fragile.

Purpose of the Study:

  • To investigate the mechanisms driving cytoskeletal disruptions during pyroptosis.
  • To clarify the functional significance of cytoskeletal element loss in pyroptosis.
  • To present immunocytochemical methods for studying cytoskeletal destruction in pyroptosis.

Main Methods:

  • Immunocytochemistry was employed to detect and assay cytoskeletal destruction.
  • Analysis of intermediate filament (vimentin) and other cytoskeletal network (microtubules, actin, nuclear lamina) integrity.
  • Assessment of cell fragility and rupture mechanisms.

Main Results:

  • Pyroptotic cells do not undergo colloid-osmotic lysis.
  • Extrinsic pressure, rather than osmotic forces, contributes to cell rupture.
  • Multiple cytoskeletal networks, including vimentin, microtubules, actin, and nuclear lamina, are lost during pyroptosis.

Conclusions:

  • Cell rupture in pyroptosis is primarily driven by cytoskeletal destabilization and fragility, not cell swelling.
  • The loss of cytoskeletal integrity is a key feature of pyroptosis, necessitating further investigation into its regulatory mechanisms.
  • Developed immunocytochemical methods facilitate the study of pyroptosis-associated cytoskeletal damage.