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Updated: Aug 2, 2025
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Automation of the Micronucleus Assay Using Imaging Flow Cytometry and Artificial Intelligence
Published on: January 27, 2023
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The Imaging Flow Cytometry-Based Cytokinesis-Block MicroNucleus (CBMN) Assay
Ruth C Wilkins1, Matthew Rodrigues2, Lindsay A Beaton-Green3
1Consumer and Clinical Radiation Protection Bureau, Health Canada, Ottawa, ON, Canada. Ruth.Wilkins@hc-sc.gc.ca.
Methods in Molecular Biology (Clifton, N.J.)
|April 19, 2023
Summary
Biodosimetry methods measure radiation exposure biomarkers in tissue. Imaging flow cytometry enhances analysis of micronuclei for rapid casualty assessment after radiological events.
Area of Science:
- Radiation biology
- Biomarker analysis
- Cellular toxicology
Background:
- Biodosimetry quantifies individual radiation doses using biological markers.
- Rapid dose assessment is crucial for medical management after radiological incidents.
- Traditional biodosimetry methods are slow and labor-intensive, limiting throughput.
Purpose of the Study:
- To review biodosimetry methods for assessing radiation exposure.
- To highlight the adaptation of assays for imaging flow cytometry.
- To focus on current methods for micronuclei identification and quantification.
Main Methods:
- Utilizing biomarkers in tissue samples to determine radiation dose.
- Adapting traditional biodosimetry assays for imaging flow cytometry.
- Employing the cytokinesis-block micronucleus assay to identify and quantify micronuclei in binucleated cells.
Main Results:
- Imaging flow cytometry offers increased sample throughput for biodosimetry.
- Current methodologies allow for efficient identification and quantification of micronuclei.
- This approach facilitates faster assessment of radiation exposure in mass casualty events.
Conclusions:
- Imaging flow cytometry significantly improves the speed and efficiency of biodosimetry.
- The cytokinesis-block micronucleus assay using this technology is a valuable tool for post-radiological event assessment.
- Enhanced biodosimetry capabilities are essential for effective medical response to radiological emergencies.
