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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Quantifying Golgi Apparatus Fragmentation Using Imaging Flow Cytometry.

Inbal Wortzel¹, Ziv Porat²

¹Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology, Drukier Institute for Children's Health, Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)

|April 19, 2023

Summary

This summary is machine-generated.

The Golgi apparatus is a dynamic organelle that changes structure in response to cellular status. We present a rapid, high-throughput imaging flow cytometry method to quantify these Golgi fragmentation changes.

Keywords:

Golgi Golgi fragmentation Golgi structure Imaging flow cytometry

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Area of Science:

Cell Biology
Organelle Dynamics
Cytometry

Background:

The Golgi apparatus is traditionally viewed as static, but it is a dynamic organelle.
It acts as a sensitive sensor of cellular status.
Golgi structure changes, including fragmentation, occur in response to various stimuli.

Purpose of the Study:

To describe a novel method for quantifying changes in Golgi architecture.
To leverage imaging flow cytometry for robust Golgi status assessment.

Main Methods:

Development and application of an imaging flow cytometry-based method.
Quantification of Golgi fragmentation (partial or complete vesiculation).

Main Results:

The described method is rapid, high-throughput, and robust.
It offers easy implementation and analysis capabilities for assessing Golgi status.

Conclusions:

The Golgi apparatus is a dynamic structure sensitive to cellular conditions.
Imaging flow cytometry provides an efficient tool for quantifying Golgi fragmentation and cellular status.