Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

60
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
60

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adsorption of metal ions by oceanic manganese nodule and deep-sea sediment: Behaviour, mechanism and evaluation.

The Science of the total environment·2023
Same author

Comparison study of supercritical water gasification for hydrogen production on a continuous flow versus a batch reactor.

Bioresource technology·2023
Same author

Passive Internet of Events Enabled by Broadly Compatible Self-Powered Visualized Platform Toward Real-Time Surveillance.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2023
Same author

Slow-light silicon modulator with 110-GHz bandwidth.

Science advances·2023
Same author

Glycerol-weighted chemical exchange saturation transfer nanoprobes allow <sup>19</sup>F<sup>/1</sup>H dual-modality magnetic resonance imaging-guided cancer radiotherapy.

Nature communications·2023
Same author

Pectic oligosaccharides ameliorate high-fat diet-induced obesity and hepatic steatosis in association with modulating gut microbiota in mice.

Food & function·2023

Related Experiment Video

Updated: Aug 2, 2025

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

11.7K

Evaluation of large-scale highly polymorphic microhaplotypes in complex DNA mixtures analysis using RMNE method.

Qiang Zhu1, Haoyu Wang1, Yueyan Cao1

  • 1West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, PR China.

Forensic Science International. Genetics
|April 19, 2023
PubMed
Summary
This summary is machine-generated.

Microhaplotypes (MHs) improve DNA mixture interpretation by enhancing the Random Man Not Excluded (RMNE) method. Using more MHs with higher discriminative power reduces interpretation errors, even with related contributors.

Keywords:
Complex DNA mixturesContributors’ relativesDNA databaseMicrohaplotypeRMNERelated contributors

More Related Videos

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

11.9K
Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
10:33

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

10.9K

Related Experiment Videos

Last Updated: Aug 2, 2025

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria
10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

11.7K
High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

11.9K
Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
10:33

Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis

Published on: June 17, 2019

10.9K

Area of Science:

  • Forensic Science
  • Genetics
  • Population Genetics

Background:

  • DNA mixture interpretation is complex, especially with multiple or related contributors.
  • Microhaplotypes (MHs) are emerging genetic markers for DNA mixture analysis.
  • Evidentiary interpretation of MH genotyping data requires further validation.

Purpose of the Study:

  • To evaluate the effectiveness of the Random Man Not Excluded (RMNE) method for interpreting mixed microhaplotype (MH) genotyping data.
  • To assess the impact of MH characteristics and mixture complexity on RMNE probabilities and non-contributor inclusion rates.

Main Methods:

  • MH loci were classified by Allele Frequency (Ae) values from the 1000 Genomes Project database.
  • Simulations of DNA mixtures with 2-10 unrelated contributors and sibling pairs were conducted.
  • RMNE probabilities were calculated for contributors and non-contributors (random men, parents, siblings), considering loci mismatch.

Main Results:

  • The number and Ae values of MHs, along with the number of contributors (NoC), influenced RMNE probabilities and non-contributor inclusion rates.
  • Increased MHs and higher Ae values, with fewer contributors, decreased RMNE probabilities and inclusion errors.
  • Kinship within mixtures significantly complicated interpretation, increasing the need for highly discriminative markers.

Conclusions:

  • Microhaplotypes show significant potential for improving mixed DNA interpretation using the RMNE method.
  • RMNE probabilities can be broadened as a parameter for DNA database searches, indicating individual relationships to mixtures.
  • Using 500 polymorphic MHs (Ae > 5) enabled distinguishing four individual types within mixtures.