Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lipid transfer protein from Manduca sexta hemolymph.

R O Ryan, M A Wells, J H Law

    Biochemical and Biophysical Research Communications
    |April 14, 1986
    PubMed
    Summary

    A lipid transfer protein (LTP) was isolated from the tobacco hornworm, Manduca sexta. This protein facilitates lipid transfer between lipoproteins in hemolymph, potentially impacting lipophorin composition.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Brain stem death induces pro-inflammatory cytokine production and cardiac dysfunction in sheep model.

    Biomedical journal·2021
    Same author

    A phase I study of binimetinib (MEK 162), a MEK inhibitor, plus carboplatin and pemetrexed chemotherapy in non-squamous non-small cell lung cancer.

    Lung cancer (Amsterdam, Netherlands)·2021
    Same author

    Endothelin receptor antagonist improves donor lung function in an ex vivo perfusion system.

    Journal of biomedical science·2020
    Same author

    Nutlin-3a Nanodisks Induce p53 Stabilization and Apoptosis in a Subset of Cultured Glioblastoma Cells.

    Journal of nanomedicine & nanotechnology·2017
    Same author

    Role of Coronary Angiography in Pre-Liver Transplantation Cardiac Evaluation: Experience From an Asian Transplant Institution.

    Transplantation proceedings·2017
    Same author

    Palliative surgical intervention in metastatic colorectal carcinoma: a prospective analysis of quality of life.

    Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2015

    Area of Science:

    • Insect physiology
    • Lipid metabolism
    • Biochemistry

    Background:

    • The tobacco hornworm, Manduca sexta, possesses hemolymph lipoproteins crucial for lipid transport.
    • Understanding lipid transfer mechanisms is vital for insect physiology and metabolism.

    Purpose of the Study:

    • To isolate and characterize a novel hemolymph lipid transfer protein (LTP) from Manduca sexta.
    • To investigate the catalytic activity and potential physiological role of the purified LTP.

    Main Methods:

    • Isolation of LTP using density gradient ultracentrifugation and gel permeation chromatography.
    • Characterization of LTP's molecular weight and apoprotein composition via SDS-PAGE.
    • In vitro assays to determine LTP's lipid transfer activity between lipoproteins.

    Main Results:

    • A purified LTP was obtained, identified as a very high density lipoprotein (Mr > 500,000).
    • LTP consists of two apoproteins: apoLTP-I (Mr ~320,000) and apoLTP-II (Mr ~85,000).
    • The purified LTP demonstrated catalytic activity for net lipid transfer between hemolymph lipoproteins in vitro.

    Conclusions:

    • The isolated LTP plays a role in mediating lipid exchange between hemolymph lipoproteins.
    • LTP may physiologically regulate the lipid content and composition of lipophorin in Manduca sexta.

    Related Experiment Videos