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Related Experiment Video

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Fluorescence detection methods for microfluidic droplet platforms
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Low-cost fluorescence microscope with microfluidic device fabrication for optofluidic applications.

Nagaraj Nagalingam1, Aswin Raghunathan1, Vikram Korede1

  • 1Process & Energy Department, Delft University of Technology, Leeghwaterstraat 39, 2628 CB Delft, The Netherlands.

Hardwarex
|April 20, 2023
PubMed
Summary

Researchers developed a low-cost optical setup for studying laser-induced cavitation and fluid flow in microchannels. This system enables detailed analysis of microbubble dynamics and fluid velocity using advanced imaging techniques.

Keywords:
ExperimentsFluorescence microscopyHigh-speed imagingLaser-induced cavitationMicrofluidics

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Area of Science:

  • Optofluidics and microscale fluid dynamics.
  • Laser-induced phenomena and bubble dynamics.
  • Development of custom optical microscopy systems.

Background:

  • Optofluidic devices enable precise fluid manipulation at micro- and millimeter scales.
  • Laser-induced cavitation offers a method for generating controlled microbubbles.
  • Characterizing microscale fluid flow is crucial for various scientific applications.

Purpose of the Study:

  • To describe a dedicated optical setup for studying laser-induced cavitation in microchannels.
  • To extend the setup for analyzing fluid flow using fluorescence-Particle Image Velocimetry (PIV).
  • To provide protocols for in-house microchannel fabrication for optical experiments.

Main Methods:

  • Utilizing a tightly focused laser beam to induce local evaporation and microbubble formation.
  • Employing high-speed microscopy and digital image analysis to track bubble interface dynamics.
  • Adapting the optical setup for fluorescence-Particle Image Velocimetry (PIV) for fluid flow analysis.

Main Results:

  • Successful generation and tracking of laser-induced microbubbles within a microchannel.
  • Demonstration of fluid flow analysis using fluorescence-PIV with minimal system modifications.
  • Development of protocols for fabricating custom microchannels as sample holders.

Conclusions:

  • The described optical setup provides a versatile platform for studying optofluidic phenomena.
  • The system allows for cost-effective, in-house construction of a fluorescence microscope.
  • This approach facilitates detailed investigation of microbubble dynamics and microscale fluid transport.