Real-time and accurate estimation ex vivo of four basic optical properties from thin tissue based on a cascade...

Double integrating sphere measurements obtained from thin ex vivo tissues provides more spectral information and hence allows full estimation of all basic optical properties (OPs) theoretically. However, the ill-conditioned nature of the OP determination increases excessively with the reduction in tissue thickness. Therefore, it is crucial to develop a model for thin ex vivo tissues that is robust to noise. Herein, we present a deep learning solution to precisely extract four basic OPs in real-time from thin ex vivo tissues, leveraging a dedicated cascade forward neural network (CFNN) for each OP with an additional introduced input of the refractive index of the cuvette holder. The results show that the CFNN-based model enables accurate and fast evaluation of OPs, as well as robustness to noise. Our proposed method overcomes the highly ill-conditioned restriction of OP evaluation and can distinguish the effects of slight changes in measurable quantities without any a priori knowledge.